human prealbumin ttr assaymax elisa kit  (Assaypro)

Journal: Nature Communications

Article Title: Sequential emergence and contraction of epithelial subtypes in the prenatal human choroid plexus revealed by a stem cell model

doi: 10.1038/s41467-025-60361-9

Figure Lengend Snippet: Dashed lines demarcate dCPEC islands. A Protocol schematic. Small ESC clumps seeded at low density and subjected only to media changes. BMP4 treatment is 15 days starting at 1 div. B Derivation milestones (phase contrast and epifluorescent TTR ICC) include small low-density ESC colonies after seeding, colony growth to 150–200 um diameter at the start of derivation (0 div), confluent islands by 5 div, and 3D islands by 35 div. C BMP4 time-of-addition (epifluorescent TTR ICC; 20 div). 15 day BMP4 application starting at 1 div, but not 0 or 2 div, leads to strong dCPEC induction. D Neural induction media (NIM) duration (epifluorescent TTR ICC; 25 div). Five days of NIM leads to better dCPEC differentiation than 15 or 25 days. E Example of an efficient derivation (epifluorescent TTR ICC; 45 div). Stitched images of one well in an 8-well chamber slide. F Marker acquisition order (confocal maximal projections; ICC): 35 div for TTR, AE2, AQP1, CLDN1; 50 div for OTX2; 70 div for CLDN5. G Apicobasal polarity (confocal and orthogonal views; ICC 45 div). Apical AQP1+ surfaces face the media, while AE2+ basolateral surfaces abut. H EdU incorporation (40 uM EdU; epifluorescent; 35 div). EdU application from 31–35 div leads to robust labeling of proliferating non-dCPECs (red), but not dCPEC islands. I Mitochondrial activity (confocal maximal projections of 150 nm MTO with TTR-ZO1 ICC; 35 div, ZO1 not shown; box plots designate medians, means “+”, 25th-75th quartiles, and min-max ranges) showing TTR+ dCPECs with significantly higher MTO levels (red) than non-dCPEC neighbors ( t -test p < 0.0001****; dCPEC n = 367 cells, non-dCPEC n = 200 cells). J TTR secretion into media following washouts (1 ug/mL BFA; ELISA; means +/- s.d.; 45 div). TTR increases in control wells (blue line) were reduced or blocked acutely by BFA at 2 h (red and green lines), then increased in the absence of BFA after secondary washouts at 5 h, indicating reversibility. Source data provided as a Source Data file. See Statistics and Reproducibility section of Methods for additional information.

Article Snippet: Longitudinally collected media were assayed using a human TTR ELISA kit (AssayPro #EP3010-1) per manufacturer protocol and read on an HT microplate reader (BioTek Synergy).

Techniques: Marker, Labeling, Activity Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: bioRxiv

Article Title: Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)

doi: 10.1101/2025.02.27.640583

Figure Lengend Snippet: Detergent effect on peptide signal in serum or purified protein digestions (A, B). Chromatograms from digestions of different matrices and purified reference protein for C) FSG and D) GSP. Concentrations of E) RBP4 and F) TTR calculated in different matrices spiked at QC concentrations. The shaded area represents 15% above or below the nominal concentration. One representative day of three is shown.

Article Snippet: These samples were analyzed by LC-MS/MS and by commercial RBP4 and TTR ELISA kits (Human RBP4 Quantikine Kit, R&D Systems, Minneapolis, MN and Human TTR ELISA Kit, Abnova, Taipei City, Taiwan).

Techniques: Purification, Concentration Assay

Journal: bioRxiv

Article Title: Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)

doi: 10.1101/2025.02.27.640583

Figure Lengend Snippet: The isotope labeled (K[ 13 C 6 15 N 2 ] or R[ 13 C 6 15 N 4 ]) internal standard peptides for RBP4 peptide (A, C, E) and for TTR peptide (B, D, F) are in grey and the corresponding analyte peptides are in black. Matrix with isotope labeled internal standard (A, B), spiked samples at lower limit of quantitation (C, D), and representative serum sample (E, F).

Article Snippet: These samples were analyzed by LC-MS/MS and by commercial RBP4 and TTR ELISA kits (Human RBP4 Quantikine Kit, R&D Systems, Minneapolis, MN and Human TTR ELISA Kit, Abnova, Taipei City, Taiwan).

Techniques: Labeling, Quantitation Assay

Journal: bioRxiv

Article Title: Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)

doi: 10.1101/2025.02.27.640583

Figure Lengend Snippet:

Article Snippet: These samples were analyzed by LC-MS/MS and by commercial RBP4 and TTR ELISA kits (Human RBP4 Quantikine Kit, R&D Systems, Minneapolis, MN and Human TTR ELISA Kit, Abnova, Taipei City, Taiwan).

Techniques: Biomarker Discovery, Control, Quantitation Assay

Journal: bioRxiv

Article Title: Development and Validation of a Novel LC-MS/MS Based Proteomics Method for Quantitation of Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR)

doi: 10.1101/2025.02.27.640583

Figure Lengend Snippet: Bland-Altman plot comparing percent difference of A) RBP4 and B) TTR measurements from LC-MS/MS and ELISA. Dotted lines: 95% CI, dashed line: mean percent bias. Deming regression for C) RBP4 ([LC-MS/MS] = 1.05*[ELISA]-0.16) and D) TTR ([LC-MS/MS]=0.94*[ELISA]+0.78), dashed line: regression, solid black line: equivalence.

Article Snippet: These samples were analyzed by LC-MS/MS and by commercial RBP4 and TTR ELISA kits (Human RBP4 Quantikine Kit, R&D Systems, Minneapolis, MN and Human TTR ELISA Kit, Abnova, Taipei City, Taiwan).

Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay